[Cloud-Clone] ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)

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Cloud-Clone의 Sandwich ELISA Kits 제품을 소개드립니다.
 

 

• Product No.:SEB792Mu
• Organism Species: Mus musculus (Mouse) Same name, Different species.
• Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Test Method: Double-antibody Sandwich
• Assay Length: 3h
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.3pg/mL.

Specificity:

• This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A8 (S100A8).
• No significant cross-reactivity or interference between S100 Calcium Binding Protein A8 (S100A8) and analogues was observed.

Recovery:

Matrices listed below were spiked with certain level of recombinant S100 Calcium Binding Protein A8 (S100A8) and the recovery rates were calculated by comparing the measured value to the expected amount of S100 Calcium Binding Protein A8 (S100A8) in samples.
 

Matrix	Recovery range (%)	Average(%)
serum(n=5)	97-105	101
EDTA plasma(n=5)	78-98	95
heparin plasma(n=5)	91-99	94

 

Precision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A8 (S100A8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A8 (S100A8) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 

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